Sorry, no. The HoldUp holds the bike by the front wheel. The "hook" that goes over the wheel needs to be placed where the fender is located.
The Two and FourTimer racks will work with fenders as the bikes are held by the frame.
Please let us know if you have additional questions by clicking here, or giving us a ring at 888-925-4621 M-F from 7 to 4 PT.